Description: Macromolecular Crystallography Protocols : Volume 1: Preparation And Crystallization of Macromolecules, Hardcover by Doublie, Sylvie (EDT), ISBN 1588292924, ISBN-13 9781588292926, Like New Used, Free shipping in the US Doublié (microbiology and molecular genetics, U. of Vermont) presents the first of two volumes dedicated to the description of laboratory protocols for macromolecular crystallography. The 13 chapters in this volume, as the subtitle indicates, are concerned with preparation and crystallization of macromolecules. The first five chapters examine the production of proteins for structural biology, specifically discussing the use of a dual affinity tag for the production of recombinant protein; the cloning, expression, and purification of yeast proteins in a medium-sized structural genomics group, baculovirus expression of integral membrane proteins, and selenomethionine substitution in bacteria and eukaryotic cells. Remaining chapters focus on crystallization and include such topics as dynamic light scattering, screening and optimization methods, the improvement of marginal crystals, high-throughput optimization, crystallization of membrane proteins, and methods for growing protein-DNA and protein-RNA crystals. The second volume explores structure determination. Annotation ©2007 Book News, Inc., Portland, OR ()
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Book Title: Macromolecular Crystallography Protocols : Volume 1: Preparation
Number of Pages: Xiv, 280 Pages
Language: English
Publication Name: Macromolecular Crystallography Protocols, Volume 1 : Preparation and Crystallization of Macromolecules
Publisher: Humana Press
Item Height: 0.3 in
Publication Year: 2007
Subject: Life Sciences / Cell Biology, Life Sciences / Biochemistry, Physics / Crystallography, Chemistry / General
Type: Textbook
Item Weight: 46.2 Oz
Author: Sylvie Doublié
Item Length: 9.3 in
Subject Area: Science
Item Width: 6.1 in
Series: Methods in Molecular Biology Ser.
Format: Hardcover
